Phenylenevinylene conjugated oligoelectrolytes as fluorescent dyes for mammalian cell imaging.

نویسندگان

  • Paulina Gwozdzinska
  • Roza Pawlowska
  • Justyna Milczarek
  • Logan E Garner
  • Alexander W Thomas
  • Guillermo C Bazan
  • Arkadiusz Chworos
چکیده

Conjugated phenylenevinylene oligoelectrolytes, which consist of a phenylenevinylene core equipped at each end with hydrophilic pendent groups, are shown to be good candidates for mammalian cell membrane staining. When used in the micromolar concentration range, they express low to moderate cell toxicity for selected regular and cancerous cell lines as tested for adherent and suspension cells.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Nanohybrid conjugated polyelectrolytes: highly photostable and ultrabright nanoparticles.

We present a general and straightforward one-step approach to enhance the photophysical properties of conjugated polyelectrolytes. Upon complexation with an amphiphilic polymer (polyvinylpyrrolidone), an anionic conjugated polyelectrolyte (poly[5-methoxy-2-(3-sulfopropoxy)-1,4-phenylenevinylene]) was prepared into small nanoparticles with exceptional photostability and brightness. The polymer f...

متن کامل

Evaluation of Chemical Fluorescent Dyes as a Protein Conjugation Partner for Live Cell Imaging

To optimize live cell fluorescence imaging, the choice of fluorescent substrate is a critical factor. Although genetically encoded fluorescent proteins have been used widely, chemical fluorescent dyes are still useful when conjugated to proteins or ligands. However, little information is available for the suitability of different fluorescent dyes for live imaging. We here systematically analyze...

متن کامل

Imaging of Cancer Cells using Gold Nanoparticels and Fluorescent Dyes by Multiphoton Microscopy

Due to the special optical properties, optical probes including metal nanoparticles (NPs), quantum dots, and fluorescent dyes, are increasingly used as labeling tools in biological imaging. Using multiphoton microscopy and fluorescence lifetime imaging (FLIM) system, we recorded the images from gold NPs conjugated to monoclonal ACT1 antibody, fluorescence dye Alexa 488 (A488) conjugated to ACT1...

متن کامل

Tuning cell surface charge in E. coli with conjugated oligoelectrolytes

Cationic conjugated oligoelectrolytes (COEs) varying in length and structural features are compared with respect to their association with E. coli and their effect on cell surface charge as determined by zeta potential measurements. Regardless of structural features, at high staining concentrations COEs with longer molecular dimensions associate less, but neutralize the negative surface charge ...

متن کامل

Coating particles with dextran-conjugated fluorescent dyes or other hydrophilic compounds.

Imaging and reconstruction of developing neurons require cells that are labeled in a way that distinguishes them from their neighbors. This can be achieved with ballistic labeling, which refers to the delivery of a cell label by means of carrier particles (tungsten or gold) propelled from a pressurized gun. Ballistic delivery can reach many dispersed cells in one shot and can deploy a wide vari...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • Chemical communications

دوره 50 94  شماره 

صفحات  -

تاریخ انتشار 2014